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biochar size experiment part 1

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Comments

Anonymous

Wow that is cool Cody Biochar smaller size does not asborb as much nutrients too much maybe.

Anonymous

Looking forward to the final results, I've wondered about particle size myself.

Anonymous

That's not the conclusion that he came to at all. He determined the chlorine in the water that he washed the charcoal with affected the experiment.

Anonymous

Not a criticism, just a tip: If you add a small light behind you or behind and over your shoulder somewhat you will light up the back of your shoulders and hair which will isolate you from the black background (love the black background!)

Anonymous

This is super interesting to me, I'm trying to grow some plants in a relatively challenging environment (near opposite of your conditions), I added a bunch of aquarium charcoal to the pots with a bunch of extra fertiliser to pre-load the charcoal because of your experiments, too soon to tell yet though, as I only did it right at the end of summer which really restricts their growth rate anyway.

Anonymous

This is a cool experiment! I'm excited to see the results. I'd be interested to see if there is any difference in root growth as well.

Anonymous

Good job in catching the chlorinated water factor as the cause of the data anomaly. I'd have just figured that pot got unlucky with the grass seeds.

Anonymous

Nice experiment! I would be curious to know what happen with an non inoculated charcoal. It could be nice to add a raw charcoal bucket on a side

Andreas Nohl

Hello, You from different locations. Is there Chlorine in Your water? Here in Germany it is only allowed for a short time if there was a damage to the system.

Andreas Nohl

You could use the Stone that you cut out with the chainsaw, as an additional sample. Is that a kind of volcanic ash sediment that hardens if it comes in contact with CO2, like some others?

Max Eliaser

In the US it's common for municipal water to be chlorinated either every summer or all year. LA's water tastes horrendous out of the tap. I feel lucky to be living in a city that doesn't chlorinate, and I grew up in a house that had its own well.

Anonymous

From what ive seen of companies that sell biochar, they use mixed variety of sizes and basically compost the charcoal for a few months before shipping it out. They say the biochar can last in the soil creating a healthy ecosystem of bacteria and other micro organisms for thousands of years.

Nani Isobel

You didn't mention where the grass seed came from. Chicken Hole Base? Using grass from there might be a indication of how well the biochar will work there.

CodysLab

Oh! I used crested wheat grass. It’s not native but is growing here.

Anonymous

Nicely done, and as usually I love your rigor, and honesty in reflecting on the shortcomings in the process. As well as the chlorine in the water, I wonder if just the additional rinsing washes away some more nutrients from the charcoal. I imagine that full bucket from the last fine rinse just teaming with nutrients.

Anonymous

Is it worth having an inoculated perlite sample as well? I recall seeing some perlite brands add fertilizer to the perlite I assume you're using the unfertilized kind, in which case I could see similar inoculation having a meaningful effect.

Anonymous

You mentioned using a larger sample size with 5 replicates per treatment. Sounds to me like you should do a randomized block design mesocosm experiment. If you (or anyone else) is interested, I could provide some excel sheets and/or R scripts with code to analyze these types of experiments to find statistically significant effects of your variables and between treatments. You could have six blocks of pots/buckets, with each block containing one replicate of each treatment, including control and a reference treatment (i.e. raw biochar, perlite,etc). So 6 replicates total. You could pry get away with two manipulated variables (e.g. particle size is one, maybe the other could be a binary factor like activated vs raw). Then all you'd need to do is run the experiment, you don't even have to move the pots around as long as their orientation in each block is randomized. Then you could take your cuttings, dry them out, and record their weights in an excel.csv file with more rows than columns (tidy form). Then all it would take is to start a new R script, read the excel csv file into R, use the function library() to load a few packages needed to run linear mixed effects models (lme4, tidyverse, emmeans, effects), and then you could analyze using a linear mixed effects model (lmer). It would look something like this: lmerCharSize <- lmer(Grass Weight ~ Char_Size * Activation_Type + (1|Block #), data = Char_Size_Dataframe). Then, use shapiro.test(resid(lmerCharSize)) to determine if the residuals (error) are normally distributed, you want p > 0.05. If the Shapiro p value is BELOW 0.05, Grass_Weight needs to be transformed using power transfrom/boxcox). Then after making sure it passes the Shapiro Test, all you'd need to do is run a few functions to look at effect sizes and statistical significance of your fixed effects (Char size and activation). These include plot(allEffects(lmerCharSize)); anova(lmerCharSize); em1 <- emmeans(lmerCharSize, ~ Char_Size * Activation), and then do a contrast(em1, method = "trt. vs ctrl", ref = Control_all sizes_char, not activated). Anova will tell you whether the variables Char_Size and Activation significantly affect grass wt, and emmeans contrast will tell you differences between each treatment and control.

Aaron

I really enjoyed this video. I liked the black background and the straight-to-the-science attitude. Also the video quality seemed great. Keep it up Cody. You’re awesome.

Anonymous

Hey Cody. In terra preta there is shards of pottery. That might be something else to try testing.

Anonymous

Hi cody Do you think lining a mask with activated charcoal is a good idea 💡 or am I wasting my time ?

CodysLab

So long as you are not breathing in carbon particles I don’t see how it would be harmful.