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In this video I show the essential steps not shown in my pan cyan video, that is, how to generate the grain spawn if all you have is a spore print.  This video was requested by a patron, hope you enjoy it.  It is also available on Rumble and on Odysee.  Using a spore syringe is an easier alternative to using agar.

Note: some people say you should wait 24 hours before using a freshly made spore syringe to give the spores time to fully hydrate.  I consider this idea debunked mythology.  Anyone who has ever done any spore work with a microscope knows the dry spores fully hydrate in minutes if not seconds, you can actually watch it happen under the microscope.  They most certainly do NOT need 24 hours.  I've made plenty of spore syringes where I did not wait at all between creating the syringe and injecting it to grain jars, and they worked perfectly.  In fact I actually RECOMMEND using them as fast as possible, why give any potential contam or bacteria a chance to grow in the water?  Just get those spores on grain as soon as possible and get it into an incubator.

[Note: If you use any of my Amazon links it supports the channel!  (“As an Amazon Associate I earn from qualifying purchases.”)]

https://amzn.to/3ABkdmP (Sterile Syringes, luer lock)

https://amzn.to/3g7wVQP (optional syringe tip caps, luer lock)

https://amzn.to/3IOdFUu (Lids)

https://amzn.to/35zcPgf (mycobags)

https://amzn.to/3IPr5Q7 (presto sterilizer)

https://midwestgrowkits.referralrock.com/l/1GORDOTEK90/  (Look under “Substrate Jars”)

Whole Oats: https://www.tractorsupply.com/tsc/product/producers-pride-oats-50-lb

Or on amazon for much higher price: https://amzn.to/3rT0NWk

https://amzn.to/34hnITg (Polyfill, cheaper at walmart or cut open a stuffed animal)

https://amzn.to/3KYHcN8 (16 oz mason jars)

https://amzn.to/3gaPYtt (hygrometer/thermometer)

I show how to make and use a still air box in this vid: https://www.patreon.com/posts/29675383

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Links to all of my TEKs, Videos, Trip music, and frequently asked questions with answers can be found here: https://www.patreon.com/posts/22774475 (everything is made available for FREE, I do not use pay walls, support the work only if you feel like you've benefited from it and you are able to do so).

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Files

Making and Using a Spore Syringe (for mycology)

YouTube deleted my old channel, this new channel will only have less controversial videos. To get access to ALL CONTENT or request a topic, please use patreon, I also post and interact with people there: https://www.patreon.com/GordoTEK Before asking a question, please read the FAQ: https://www.patreon.com/posts/22774475 Social media:  https://twitter.com/TekGordo  https://www.facebook.com/gordo.tek.33  [Note: If you use any of my Amazon links it helps support the channel! (“As an Amazon Associate I earn from qualifying purchases.”) I make zero from YouTube. Thanks!] https://amzn.to/3ABkdmP (Sterile Syringes, luer lock) https://amzn.to/3g7wVQP (optional syringe tip caps, luer lock) https://amzn.to/3IOdFUu (Lids) https://amzn.to/35zcPgf (mycobags) https://amzn.to/3IPr5Q7 (presto sterilizer) https://midwestgrowkits.referralrock.com/l/1GORDOTEK90/  Look under “Substrate Jars” Whole Oats: https://www.tractorsupply.com/tsc/product/producers-pride-oats-50-lb Or on amazon for much higher price: https://amzn.to/3rT0NWk https://amzn.to/34hnITg (Polyfill) https://amzn.to/3KYHcN8 (16 oz mason jars) https://amzn.to/3gaPYtt (hygrometer/thermometer) I show how to make and use a still air box in this vid: https://www.patreon.com/posts/29675383

Comments

Anonymous

Thank you for sharing your tuition and experience. I would love a spore print.

Wazatron

Does using a laminar flow hood completely eliminate the need to work in a still air box? I believe so, but I'm hoping to check my work to make sure I'm not making a silly mistake planning things out.

Kopo Red

No for instance if you have a agar plate with contamination. And want to clean it up. You must use a SAB. A flow hood can de disastrous in this situation.