Home
Artists
Search Recent Random
Posts
Search DMs Popular Hash Lookup Tags Random
Importer
Import FAQ
Register
Partychan Telegram ThePornDude
Home Artists Posts Import Register

New vid: Making and Using Agar (Patreon)

Published:
2021-09-15 22:59:30
Imported:
2023-10

Content

In this video I show how to make and use agar.  Thought it would be a good idea to put this vid out before publishing the Pan Cyan TEK vid which I've been working on for far too long (I know).  I want to give away lots of spore prints but only if people know what to do with a spore print ;) Hopefully this vid makes it clear.  This video is also on Rumble and on Odysee.  Note that making a spore syringe may be a better alternative for beginners (I created a video showing that as well)

Links to all vids banned by YouTube can be found here.  

Social Media: FB and Twitter  

[Note: If you use any of my Amazon links it supports the channel!  (“As an Amazon Associate I earn from qualifying purchases.”)]

Petri dishes 

Inoculation loops 

Xacto Knife  (for scraping spores and cutting agar)

Premixed MEA powder (not recommended but for the lazy)

To make your own mix: Light Malt Extract

Agar Agar Powder or this one  

Lighters for flame sterilization 

Good value blender or Blendtec   

Large pressure cooker (great value and good reviews)  or smaller one 

Kitchen Scale (great reviews, inexpensive)

Large plastic funnel with strainer insert 

Optional parafilm but ziplock bags work fine.

Optional stuff only if making PDA or black agar: Honey and Activated Carbon

Music Credit to Gil Scott Heron - The Revolution Will Not Be Televised "The thing that's going to change people is something that no-one will ever be able to capture on film, it will just be something you see and all of the sudden you realize I'm on the wrong page..." -Gil Scott Heron (explaining the meaning of his song). 

Don't forget to follow me on Patreon and request a free spore print if you haven't already received one and could use it.  My next video will NOT be on YouTube.

______________________________________

Links to all of my TEKs, Videos, Trip music, and frequently asked questions with answers can be found here: https://www.patreon.com/posts/22774475 (everything is made available for FREE, I do not use pay walls, support the work only if you feel like you've benefited from it and you are able to do so).

Files

Making and Using Agar (for mycology)

YouTube deleted my old channel, this new channel will only have less controversial videos. To get FREE access to ALL CONTENT and a free spore print, please use patreon, I also post and interact with people there: https://www.patreon.com/GordoTEK Before asking a question, please read the FAQ: https://www.patreon.com/posts/22774475 Social Media Links: https://www.facebook.com/gordo.tek.33 https://twitter.com/TekGordo In this video I show you how to make and use agar. Written up TEK is here: https://www.patreon.com/posts/56209839 Recommended LMEA recipe: 8 grams of light malt extract 8 grams of agar powder 400ml hot water [Note: If you use any of my Amazon links it helps support the channel! (“As an Amazon Associate I earn from qualifying purchases.”) I make zero from YouTube. Thanks!] Petri dishes: https://amzn.to/2Xgvauu Inoculation loops: https://amzn.to/3CcX1uB Xacto Knife: https://amzn.to/3ltxYwv Premixed MEA powder (not recommended but for the lazy): https://amzn.to/3AbcTNF To make your own mix: Light Malt Extract: https://amzn.to/3k9ao8Q Agar Agar Powder: https://amzn.to/3AdiJ14 Lighters for flame sterilization: https://amzn.to/397qmu8 Good value blender http://amzn.to/2Dsq5QR Blendtec Blender https://amzn.to/2XjhBdu Large pressure cooker (great value and good reviews): https://amzn.to/2Fsbfgr or smaller one: https://amzn.to/399EX8n Kitchen Scale (great reviews, inexpensive) http://amzn.to/2BobRP0 Large plastic funnel with strainer insert: https://amzn.to/2RDBzeQ Optional parafilm: https://amzn.to/3CcZhC9 But ziplock bags work fine: https://amzn.to/3lp70Go Optional stuff only if making PDA or black agar: Honey: https://amzn.to/3k880zm Activated Carbon: https://amzn.to/3EkTufx Music Credit to Gil Scott Heron - The Revolution Will Not Be Televised https://youtu.be/QnJFhuOWgXg "The thing that's going to change people is something that no-one will ever be able to capture on film, it will just be something you see and all of the sudden you realize I'm on the wrong page..." -Gil Scott Heron (explaining the meaning of his song) Don't forget to follow me on Patreon and get your free spore print. My next video will NOT be on YouTube. Recipe cards from this video can be found here: https://www.patreon.com/posts/56209839

Comments

Timothy Kramer

I have the products ordered and building a hood. Thanks for sending me a print and all your helpful instructions. I see you have a Kill-A-Watt connected to the heater. I use a Kill-A-Watt to testing how much juice a device is pulling, is that why you use it with the aquarium heater? Also I've used a Instant Pot to prep grain jars and have had success. It reaches a couple point short of 15PSI. Any thoughts on using a Instant Pot. I will do the agar in it and will let you know how it turns out. Should I Run blank plates to test? I have cube prints also I can run as a test before using your print.

Anonymous
>>105989322

@Timothy Kramer You can use an Instant Pot for both grain spawn and agar with no problem. For agar, transfer your media jar to your still air box or flow hood while still hot. If your sterile procedure is good, you'll have contamination free plates.

Dr. Anteater

@gordotek in your video you pour 2-3 dishes out of each pint jar. How long can those jars keep and still be viable, and what is your process for unused agar?

JB
>>116002556

Throwing my 2 cents of very little experience in trying to re-use unused agar. i've tried: 1. re-pressure-cook it 2. microwave it. Result = i could not get it to become somewhat liquid again. it remained gelatinous and therefore unusable. I'm remaking agar from scratch, just halving the ingredients.

Mario Nardoni

@gordotek I am brand new to mycology and I am finding your channel very interesting and helpful. I have couple questions. I recently purchased some spore siringes, my question is what is the different between spore siringes and culture siringes? If there is a difference what is the process to turn spores into culture? The second questions. by mistake I ordered a spore print instead of a spore siringe, I followed instructions on how to inoculate an agar petri dish with spores. I now have nice little round fluffy mycelium growths, but each has a greenish dot on the center. Is this normal or is any greenish stuff a bad thing?